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Primer Design Guidelines

The following recommendations are provided to help optimize primer selection:

  • Use of primers longer than 18 bases also minimizes the chance of having a secondary hybridization site on the target DNA.
  • Avoid runs of an identical nucleotide, especially guanine, where runs of four or more Gs should be avoided.
  • Keep the G-C content in the range of 50-55%.
  • For primers with a G-C content less than 50%, it may be necessary to extend the primer sequence beyond 18 bases to keep the Tm>55oC.
  • Avoid primers that have secondary structure or that can hybridize to form dimers.

For each reaction requested, please provide 3 uL of primer at a concentration of 5 pmol/uL (uM). 

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MMAS Contact Information

Physical Address
MOLB Molecular Analysis Lab
Chemistry & Biochemistry Bldg. W357
New Mexico State University
1175 N Horseshoe St
Las Cruces, NM 88003-8001

Mailing Address:
MOLB Molecular Analysis Lab
New Mexico State University
MSC 3MLS P O Box 30001
Las Cruces, NM 88003-8001