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New Mexico State University

Sample Submission Guidelines

Please submit a completed ABI Sequencing Sample Form with your samples.

Sample Requirements

Template

Concentration

Volume

Plasmids

200 ng/ul

12 ul minimum

PCR products

5 ng/ul per 100 bp

12 ul minimum

Primer

5 uM

 

  • Please provide enough template for repeats in case of technical failure.
  • Please provide 3 uL of primer at a concentration of 5 uM (pmol/uL) for each reaction requested.
  • We will provide standard vector sequencing primers without extra charge.  Available primers:  M13 Forward, M13 Reverse, T7, T3, Sp6, T7 terminator, TriplEx.
  • The templates and primers are stored in the -20oC freezer for up to 3 months from the time of submission. à Customers can pick-up the leftover templates and primers after the completion of sequencing reactions. 

Sample Drop Off

  • Sequencing reactions are expected to start around 9:00 am Wednesdays and Fridays. 
  • Please drop your samples in the sequencing refrigerator and leave your completed Sample Form on the director's desk. 

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MMAS Contact Information

Physical Address
MOLB Molecular Analysis Lab
Chemistry & Biochemistry Bldg. W357
New Mexico State University
1175 N Horseshoe St
Las Cruces, NM 88003-8001

Mailing Address:
MOLB Molecular Analysis Lab
New Mexico State University
MSC 3MLS P O Box 30001
Las Cruces, NM 88003-8001